Embryology Laboratory (BIO 309)

Fall 2005

Wed - 2:20-5:20 - 102 Gravely Science Building

 

INSTRUCTOR:           Darryl L. Daley, Ph.D.

 

OFFICE:                       107A Gravely Science Building

 

PHONE:                       985-5235  - (E-Mail - ddaley@ncwc.edu)

 

OFFICE HOURS:        Mon - 2:20-3:20

                                      Tue - 1:30-2:30

                                      Thur - 9:30 AM-10:30 AM

                                      Fri - 2:20 PM-3:20 PM

                                      Also by appointment

 

Web site:                   http://faculty.ncwc.edu/Ddaley/

 

REQUIRED

TEXTS:                         Wright, Shirley J. 2005. A Photographic Atlas of Developmental  Biology. Morton Publishing Company, Englewood, Colorado.

GOALS:

 

1. Be able to identify and explain chromosome activity during the major stages of mitosis, oogenesis and spermatogensis in insects, amphibians and mammals.

 

2. Be able to identify the basic developmental stages during cleavage, blastulation, gastrulation and neurulation in invertebrates and amphibians.

 

3. Be able to work with living fish embryos and describe the developmental stages from cleavage to hatching.

 

4. Be able to identify early developmental stages in chick embryos and work with living chick embryos.

 

5. Be able to describe organogensis if major body systems in a mammal.

 


 

Evaluation:                                 

                                            Practical on Labs 1-5                                    25%                             250

                                            Practical on Labs 6-10                                  25%                             250

                                            Assignments and quizzes                           45%                             450

                                            (50 points per lab)

                                            Attendance                                                    5%                                50

                                           

 

                                            TOTAL POINTS POSSIBLE                    100%                         1000

 

 

 

GRADING WILL BE ON A STRAIGHT PERCENT BASIS:

 

93% or Greater: A       76 - 79%: C+

90 - 92%     : A-             73 - 75%: C

86 - 89%     : B+            70 - 72%: C-

83 - 85%     : B               65 - 69%: D+

80 - 82%     : B-             60 - 64%: D

      Less than 60%: F

 

 

Attendance Points

 

Students with perfect attendance for the entire semester will earn 50 points of attendance credit.  If you miss one lab, you will earn only 25 points.  In addition, by missing the lab you will not earn the points associated with the lab (50 points).  Thus by missing a single lab a B grade would drop to a C+.  

 

 

Exam Policy

 

No make-up exams will be given, but see the professor with a documented excuse in extreme situations

 

 

ATTENDANCE

 

Attendance And Punctuality At All Laboratory Meetings Is Required!

 

If you are absent from a lab session you will not be allowed to attend a different section's lab.  If you are absent twice from your lab section, you will be withdrawn from the class, resulting in either a "W" or "F" depending on the date withdrawn.  If you miss a lab for an unavoidable reason (e.g. a funeral, emergency hospitalization) and it is documented (e.g. hospital bill), please let the professor know (ahead of time if possible), and the professor will allow you to make up the lab or allow you to attend another section.  A note from the Colleges nurse does not permit you to miss a lab due to illness unless the nurse provides you with documentation stating specifically that you should not be attending class.  If you are late for lab three times, this will count as one absence, and you will lose 25 of your attendance points as if you had missed one entire lab.  If a quiz was given at the beginning of lab and you are late for the quiz, you will not be allowed to take the quiz.

 

 

Academic Integrity

 

Working with each other in study groups is encouraged and is a great way of learning, but each student is to write his or her own assignments individually; they are not to be written with another student.  Refer to the catalog (p 78-79) for College's policy on plagiarism and cheating.

 

 

Laboratory Schedule

 

Date                                                                Topic

 

08/24             Lab 1  Mitosis & Oogenesis in rabbit and frog ovary (ADB, Pages 67 & 68; Pages

39 - 52)

 

08/31               Lab 2  Spermatogenesis in grasshopper testis, frog testis, rat testis, human &

other sperm cells (ADB Pages  17 - 38)

 

09/07               Lab 3  Cleavage in sea urchins, starfish and frogs

                        (ADB Pages 67 - 85)

 

09/14               Lab 4  Fertilization and early development living sea urchins

                        Note: Lab times dependent upon arrival of sea urchins (ADB Pages 53 - 66)       

           

09/21               Lab 5  Blastulation and gastrulation in starfish and frogs

                        (ADB Pages 87 - 104)

 

10/28               Lab 6  Neurulation and 4, 7 and 10 mm frog embryos

                        (ADB Pages 105 - 128)

 

10/05               Practical on Labs 1-6

 

 

10/12               Lab 7  Japanese Medaka - live animals - Early stages & organogenesis

                         Note: Lab times are dependent upon arrival of Medaka

                       

10/19               Lab 8  Japanese Medaka - live animals - Environmental effects experiment

                        Note: No regular lab time - students will work in groups on individual

experiments

 

10/26               Lab 9  Live Chick embryos & Prepared slides on Avian organogenesis -  24 or 28,

48, 72 hr and 96 hr (ADB Pages 171 - 226)

 

11/02               Lab 10            Finish Avian organogenesis & Amphibian organogenesis (ADB Pages 143 -

170)

             

11/09               Lab 11 Porcine organogenesis, 10mm embryos

                        (ADB Pages 227 - 270)

 

11/16               Practical on Labs 7-11

 

11/23               No Lab  - Thanksgiving Break

 

11/30               No Lab

 

ADB = A Photographic Atlas of Developmental Biology. 2005. Shirley J. Wright. Morton Publishing Company. Englewood, Colorado.

                       

 

Laboratory Safety

 

·        Absolutely no food, drinks, gum, hard candy or cough drops in the lab.

·        If you hurt or cut yourself, let the professor know immediately even if it seems minor.

·        Dispose of any broken glass (slides, etc.) in the "Glass Disposal" box.

·        Wearing long pants and shoes instead of shorts and scandals will help protect you from any chemical spills or broken glass.

·        Stains and dyes will be used in the lab; you may not want to wear your best clothes.

·        Please let the professor know if you have health concerns, such as allergies, so that proper precautions can be taken.

 

Remember

 

·        You are required to read each lab before you come to lab.

·        For some labs there will be pre-lab assignments or quizzes that will be completed before doing the lab exercise.

·        You are responsible for cleaning your work area before you leaving the lab.

·        We will be studying preserved animals this semester in lab.  Please treat the specimens with respect and act responsible when handling them.

 

Note:

 

Students with disabilities who believe that they may need accommodations in this class are encouraged to contact the Office of Disability Support Services (DSS) at 985-5369 as soon as possible to coordinate and implement accommodations in a timely fashion. The Office of DSS is located in the Student Support Center, PC 188.